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A molecule of double-stranded DNA was cleaved with restriction nucleases, and the resulting products were separated by gel electrophoresis (Figure Q 10-11 ). DNA fragments of known sizes were electrophoresed on the same gel for use as size markers (left lane). The size of the DNA markers is given in kilobase pairs (kb), where 1 kb= 1000 nucleotide pairs. Using the size markers as a guide, estimate the length of each restriction fragment obtained. From this information, construct a map of the ori
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SOLVED: The table shows where different restriction endonucleases (restriction enzymes cleave DNA. The abbreviation R represents the purines adenine and guanine.The pyrimidines cytosine,thyminc,and uracil are abbreviated as Y.The abbreviation W represents adenine orthymine. Enzyme Target sequence Cleavage 5'GAATTC3' EcoRI 5'G AATTC3 3'CTTAAG5 3CTTAA GS' S'GATATC3 EcoRV 5GAT ATC3' 3CTATAG 3'CTA TAGS' 5GGCC3 5GG CC3 HaelII 3CCGG5 3CC GG5 5AAGCTT3 S'A AGCTT3 HindIII 3TTCGAA5 3TTCGA AS' 5'RGGWCCY3 S
SOLVED: The table shows where different restriction endonucleases (r…
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