SOLVED: A scientist wants to study the kinetics of a specific membrane protein. They ligate the DNA coding region of the protein to the DNA coding region for GFP and introduce the DNA into cells. A day or two later, they observe the cells under the microscope and they are fluorescing green. They then shine a laser at a region of the cell repeatedly (or continuously) - that region loses its fluorescence. They monitor a separate region of the cell and find that fluorescence never decreases in that
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LB-amp: nutrient agar with ampicillin and X-gal added to the media (colonies of interest). Genomic ligation: cut yeast DNA with cut plasmid and ligate into one recombinant plasmid (Add recombinant into bacterial cell). The result would be a combination of both recombinant plasmids and non-recombinant plasmids. Conditions 1, 2, and 3 are represented in this population of cells. Plate out these three different types of cells onto media containing ampicillin and X-gal. The result - Blue and White C
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