Spectrophotometer: Principle, Parts, Types, and Uses • Microbe Online
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2. A spectrophotometric method for the quantitative determination of the concentration of Pb^2 inblood uses Cu^2 as an internal standard. A solution was prepared by combining 2.50mL of 7.000ppb Pb^2 with 1.000mL of 22.50ppb Cu^2 the mixture was then diluted to 10.00mL. Which give an absorbancesignal of 3.20 for lead and 1.35 for the copper. A 5.00mL sample of blood with unknown concentrationof Pb^2 is spiked with 1.000mL of 22.5ppb Cu^2 and diluted to 10.00mL the resulting solution gave asi
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