#sicklecell #sicklecellawareness #knowyourstatus #globalhealth #genetics #hydroxyurea #healthequity #dro | Lola O.
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You isolate a new cdc mutant in budding yeast and call it cdc104. You arrest cdc104 at its restrictive temperature of 37°C for 2 hours (the length of a cell cycle in yeast is normally about 90 minutes). You then release the phenotypic arrest by shifting the cdc104 mutant arrested yeast to the permissive temperature of 20°C, but you release in the presence of hydroxyurea. Hydroxyurea arrests cells in S phase because it blocks DNA replication. The cells refuse to divide. Now you reverse the orde
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